The Effect of Solvents to Extract Phenol and Phenol Related Compounds Contents and Some Biological Screening of Salvia officinalis

  • Mona A M Abdelmageed Department of Pharmacognosy, Faculty of Pharmacy, Elsheikh Abdallah University
Keywords: Saliva officinalis, Polyphenols, Flavonoids, Tannin, Antioxidant Activity, Anticandidal Activity, Topoisomerase I, Topoisomerase II.

Abstract

Saliva officinalis (Lamiaceae) herbal plant collected from Khartoum local market. This study has three aims, to assess the efficacy of three aqueous organic solvents to extract the phenol and phenol derivative metabolites using the maceration method, to assess the antifungal and anticancer of Saliva officinalis aqueous extracts using Yeast – Based bioassay against three mutant yeast strains Saccharomyces cerevisiae, and to assess the antioxidant activity of Saliva officinalis extracts using two methods: DPPH radical scavenging assay, and TLC Autography technique. The results revealed that the 70% methanol has high total phenols (318.35mg GAE/g) and total flavonoids (189.38mg QE/g) while the 70% acetone extract was revealed high total tannin content (218.34 mg TAE/g). The 70% acetone extract revealed activity against the three mutant yeast tested with inhibition zone diameter more than 12 mm at concentration 5mg/ml and 10mg/ml respectively. While the other extracts were exhibited no activity against the three mutant strains tested. All extracts were reflected anti-Candida activity and the maximum IZD was exhibited by the 70%ethanol extract (30mm). All extracts were free-radical inhibitors but 70% methanol extract was more potent (0.068mg/ml) than the other extracts.

Published
2020-01-02